InterviewSolution
Saved Bookmarks
InterviewSolution
| 1. |
Explain briefly: (i) RNA dependent DNA polymerase (ii) Isoelectric focussing (iii) Cosmids (iv) Vitamins |
|
Answer» (i) RNA-dependent DNA polymerase enzyme is used for the synthesis of a complementary DNA strand from RNA molecule as a template. It is produced by HTV and other retroviruses which help them to synthesize DNA from their viral RNA. (ii) Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different molecules by their electric charge differences. It is a type of zone electrophoresis, usually performed on proteins in a gel. (iii) Cosmids are constructed by uniting some part of the bacterial plasmids with a ‘ori’ gene, an antibiotic selection marker and a cloning site with one or more recently two ‘cos’ sites derived from bacteriophage lambda. (iv) A vitamin is an organic compound required as a nutrient in very small amounts by an organism. They act as co-factor of an enzyme. It cannot be synthesized in sufficient quantities by an organism, and must be obtained from the diet. |
|