Explain different types of restriction enzymes?

1.	Explain different types of restriction enzymes?
Answer» Different types of restrictions enzymes are as follows: 1. Type I – They function . simultaneously as endonuclease and methylase e.g. EcoK. 2. Type II – They exhibit separate cleaving and methylation activities. They are more stable and are used in r-DNA technology e.g. EcoRI, Bgll. They cut DNA at specific sites within the pallindrome. Thousands of type II restriction enzymes have been discovered. 3. Type III – They cut DNA at specific nonpalindromic sequences e.g. Hpal, MboII.

Answer»

Different types of restrictions enzymes are as follows:

1. Type I – They function . simultaneously as endonuclease and methylase e.g. EcoK.

2. Type II – They exhibit separate cleaving and methylation activities. They are more stable and are used in r-DNA technology e.g. EcoRI, Bgll. They cut DNA at specific sites within the pallindrome. Thousands of type II restriction enzymes have been discovered.

3. Type III – They cut DNA at specific nonpalindromic sequences e.g. Hpal, MboII.

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