1.

Explain the different steps involved in translation.

Answer»

Translation in evkaryotes : 

  • Charning of tRNA (amninoacylation of tRNA) : activation of animo acids in the presence of ATP and their linking to specific tRNA. 
  • Binding of the ribosome (small sub unit) to mRNA at the initiator codon (AUG) 
  • Binding of the initaitor tRNA carrying to amino acid (methionine) to the initiator codon to initiate protein synthesis. 
  • Movement of ribosome from codon to codon along the mRNA and adding of amino acids (linked to tRNA) one by one – elongation of polypeptide chain. 
  • Binding of the release factor to the stop codon located at the 3’ end of mRNA, terminating transulation and releasing the polypeptide from ribosome.


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