1.

Explain the role of RNA polymerase in transcription in bacteria.

Answer»

- A single DNA dependent RNA polymerase catalyses the formation of mRNA, tRNA 5 and rRNA in bacteria.

- The enzyme is capable of catalyzing only the elongation step of transcription 

- It combines transiently to the initiation or sigma factor and binds to the promoter and initiates transcription

- It somehow facilitates the opening of the DNA helix and catalyses the polymerisation of ribonucleoside triphosphates in a template-depended fashion, i.e., elongation. 

- When it reaches the terminator sequence, the enzyme associates transiently with the termination or rho (p) factor and terminates transcription, the RNA and the enzyme fall off the template.



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