Explain the steps involved in the production of genetically engineered

1.	Explain the steps involved in the production of genetically engineered insulin. Why is insulin thus produced preferred to the one produced from non-human sources?
Answer» Genetically engineered insulin: Production of insulin by rDNA techniques was achieved by an American company, Eli Lilly, in 1983. It prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them in plasmids of E.coli for production. The A and B chains produced were separated, extracted and combined by creating disulfide bonds to form human insulin. Since insulin produced from non-human sources causes allergy or other reactions to the foreign protein, genetically engineered insulin is preferred.

Explain the steps involved in the production of genetically engineered insulin. Why is insulin thus produced preferred to the one produced from non-human sources?

Answer»

Genetically engineered insulin: Production of insulin by rDNA techniques was achieved by an American company, Eli Lilly, in 1983. It prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them in plasmids of E.coli for production. The A and B chains produced were separated, extracted and combined by creating disulfide bonds to form human insulin.

Since insulin produced from non-human sources causes allergy or other reactions to the foreign protein, genetically engineered insulin is preferred.

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