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Explain three steps involved in polymerase chain reaction. |
Answer» Three steps involved in polymerase chain reaction : `*` Denaturation : Typically at about `93-95^(@)`C for human genomic DNA. DNA are separated by heat denaturation. `*` Primer annealing : In this step primer is attached to the complementary strands on the single stranded DNA. `*` DNA synthesis : DNA polymerase extends the primers and forms double stranded DNA. |
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