First step in Southern blot technique isA. Digestion of DNA by restriction enzymeB. Production of a group of genetically identically cellsC. Denaturation of DNA on the gel for hybridisation with specific probeD. Denaturation of DNA from a nucleated cell as from the scene of crime

First step in Southern blot technique isA. Digestion of DNA by restric

1.	First step in Southern blot technique isA. Digestion of DNA by restriction enzymeB. Production of a group of genetically identically cellsC. Denaturation of DNA on the gel for hybridisation with specific probeD. Denaturation of DNA from a nucleated cell as from the scene of crime
Answer» Correct Answer - A

Discussion

No Comment Found

Related InterviewSolutions

Crystal of Bt toxin produced by some bacteria do not kill the bacteria becauseA. Bacteria are resistant to the toxinB. Toxin is inactiveC. Toxin is immatureD. Bacteria enclose toxin is a special sac.
Crystals of Bt toxin produced by some bacteria do not kill the bacteria themselves becauseA. Bacteria are resistant to the toxinB. Toxin is immatureC. Toxin is inactiveD. Bacteria enclose the toxin in special sac.
Which technique is used in separating fragments of DNAA. Eastern blottingB. Western blottingC. Northern blottingD. Southern blotting
Endonucleases break DNA at specific sites calledA. Palindromic sequencesB. Restriction sitesC. Conserved sitesD. Both A and B
Select the wrong statementA. Presence of chromogenic substrate gives blue colour colonies if the plasmid in the bacteria does not have an insertB. Retroviruses in animals have the ability to transform normal cells into cancerous cellsC. In microinjection, cells are bombarded with high velocity microparticles of gold or tungsten coated with DNAD. Since DNA is a hydrophilic molecule it cannot pass through cell membranes
Fragments of DNA formed after treatment with endonucleases are separated by the techniqueA. Polymerase chain reactionB. Southern blottingC. Colony hybridisationD. Electrophoresis
Select the wrong statementA. Human insulin is being commercially produced from a transgenic species of Escherichia coliB. Genetically modified Bacillus thuringiensis is used as biopesticide on commercial scaleC. Human protein, `alpha`-1-antitrypsin, is used to treat emphysemaD. Bt toxin genes cry IAC control corn borer
In insectional inactivation of `beta`-galactosidase gene, the bacteria in white colonies haveA. Nonrecombinant plasmidB. Recombinant plasmidC. No plasmidD. Linear foreign DNA
Restriction endonucleases areA. Synthesized by bacteria as part of their defence mechanismB. Used in vitro DNA synthesisC. Present in mammalian cells to degrade DNA when the cells dieD. Used in genetic engineering for ligation of two DNA molecules.
DNA fragments generated by restriction endonucleases in a chemical reaction can be separated byA. Restriction mappingB. CentrifugationC. Polymerase chain reactionD. Agarose gel electrophoresis

First step in Southern blot technique isA. Digestion of DNA by restriction enzymeB. Production of a group of genetically identically cellsC. Denaturation of DNA on the gel for hybridisation with specific probeD. Denaturation of DNA from a nucleated cell as from the scene of crime

Discussion

No Comment Found

Related InterviewSolutions

Reply to Comment