Genetic Engineering include creation of recombinant DNA with the help

1.	Genetic Engineering include creation of recombinant DNA with the help of restriction enzymes.(a) Explain reombinant DNA technology.(b) What are restriction enzymes? Name a restriction enzyme.
Answer» (a) Recombinant DNA technology involves several steps. They are (1) Isolation of the Genetic Material (DNA) (2) Cutting of DNA at Specific Locations (3) Amplification of Gene of Interest using PCR (4) Insertion of Recombinant DNA into the Host Cell /Organism (5) Obtaining the Foreign Gene Product (6) Downstream Processing (b) Restriction enzyme is used to cut DNA at specific nucleotide sequence Examples are EcoR1 ,Hind111 etc.

Genetic Engineering include creation of recombinant DNA with the help of restriction enzymes.(a) Explain reombinant DNA technology.(b) What are restriction enzymes? Name a restriction enzyme.

Answer»

(a) Recombinant DNA technology involves several steps.

They are

(1) Isolation of the Genetic Material (DNA)

(2) Cutting of DNA at Specific Locations

(3) Amplification of Gene of Interest using PCR

(4) Insertion of Recombinant DNA into the Host Cell /Organism

(5) Obtaining the Foreign Gene Product

(6) Downstream Processing

(b) Restriction enzyme is used to cut DNA at specific nucleotide sequence

Examples are EcoR1 ,Hind111 etc.