Give an account of the Blue-White Method of selection of recombinants. – InterviewSolution

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1.	Give an account of the Blue-White Method of selection of recombinants.
Answer» The Blue-White Method of selection of recombinants: Based on insertional inactivation the foreign gene is inserted into Lac Z gene coding for Beta-galactosidase enzyme which are used as selectable markers. The Betagalactosidase converts colourless substrate (X gel) into blue coloured product. In transformed hosts, Lac Z is activated due to insertion of foreign DNA. Nontransformed colonies convert colourless X gel (chromogenic substrate) into blue coloured product. The transformed colonies remain white or colourless. It helps to differentiate between transformant or recombinant and non-transformant or nonrecombinant.

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