1.

Given below is the diagram of agarose gel kept under UV light :(a) Mark the positive and negative terminals. (b) What is the charge carried by DNA molecule and how does it help in its separation ? (c) How are the separated DNA fragments finally isolated ?

Answer»

(a) Positive terminal- 'B' 

Negative terminal- 'A' 

(b) DNA being negatively charged, moves towards the positive electrode (anode) 

(c) By elution-separated bands of DNA are cut out from the agarose gel and extracted from the gel piece.



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