How are transgenic tobacco plants produced to solve this problem?

1.	How are transgenic tobacco plants produced to solve this problem?
Answer» Transgenic tobacco plants are produced in following way: (i) To prevent the roots of tobacco plants from infections a strategy was adopted based on the process of RNA interference (RNAi). (ii) Nematode-specific genes were introduced into the host plants using Agrobacterium vectors. (iii) The introduction of DNA was such that it produced both sense and anti-sense RNA in the host cells. (iv) These two RNAs being complementary to each other formed a double stranded RNA (dsRNA) that initiated RNAi and thus, silenced the specific mRNA of the nematode. (v) The parasite could not survive in a transgenic host expressing specific interfering RNA, thus plant got itself protected from the parasite.

Answer»

Transgenic tobacco plants are produced in following way:

(i) To prevent the roots of tobacco plants from infections a strategy was adopted based on the process of RNA interference (RNAi).

(ii) Nematode-specific genes were introduced into the host plants using Agrobacterium vectors.

(iii) The introduction of DNA was such that it produced both sense and anti-sense RNA in the host cells.

(iv) These two RNAs being complementary to each other formed a double stranded RNA (dsRNA) that initiated RNAi and thus, silenced the specific mRNA of the nematode. (v) The parasite could not survive in a transgenic host expressing specific interfering RNA, thus plant got itself protected from the parasite.

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