1.

How aseptic conditions are maintained in tissue culture?

Answer»

1. Glassware is sterilized by using detergents and hot air oven. 

2. Nutrient medium is autoclaved under constant pressure of 15 lb/sq inch, continuously for 20 minutes to sterilize it. 

3. Explant is treated with 20% ethyl alcohol and 0.1% HgCl2

4. Sterilization of inoculation chamber (Laminar air flow) is done using UV ray tube for 1 hour before actual inoculation of explant on the sterilized nutrient medium.



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