Saved Bookmarks
| 1. |
How aseptic conditions are maintained in tissue culture? |
|
Answer» 1. Glassware is sterilized by using detergents and hot air oven. 2. Nutrient medium is autoclaved under constant pressure of 15 lb/sq inch, continuously for 20 minutes to sterilize it. 3. Explant is treated with 20% ethyl alcohol and 0.1% HgCl2. 4. Sterilization of inoculation chamber (Laminar air flow) is done using UV ray tube for 1 hour before actual inoculation of explant on the sterilized nutrient medium. |
|