How did an American Company, Eli Lily use the knowledge of r-DNA tech

1.	How did an American Company, Eli Lily use the knowledge of r-DNA technology to produce human insulin ?
Answer» Two chains of DNA sequence corresponding to A and B chains of human insulin prepared, introduced them into plasmids of E. coli to produce separate A and B chains, A and B chains extracted combined by creating disulphide bonds. Detailed Answer : American Company Eli Lilly used the knowledge of r-DNA technology to produce human insulin in the following way. 1. Preparation of DNA corresponding to A and B chain separately. 2. Extraction of plasmid from bacteria. 3. Insertion of DNA corresponding to A and B chain separately in plasmid. 4. Transforming the bacteria with this recombinant plasmid. 5. Expression of the desired product from this DNA . 6. Purication of A and B chain. 7. Linking them with disulde bonds so that they act as human insulin.

How did an American Company, Eli Lily use the knowledge of r-DNA technology to produce human insulin ?

Answer»

Two chains of DNA sequence corresponding to A and B chains of human insulin prepared, introduced them into plasmids of E. coli to produce separate A and B chains, A and B chains extracted combined by creating disulphide bonds.

Detailed Answer :

American Company Eli Lilly used the knowledge of r-DNA technology to produce human insulin in the following way.

1. Preparation of DNA corresponding to A and B chain separately.

2. Extraction of plasmid from bacteria.

3. Insertion of DNA corresponding to A and B chain separately in plasmid.

4. Transforming the bacteria with this recombinant plasmid.

5. Expression of the desired product from this DNA .

6. Purication of A and B chain.

7. Linking them with disulde bonds so that they act as human insulin.

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