How did Eli Lilly synthesise the human insulin? Mention one difference between this insulin and the one produced by the human pancreas.ORHow did Eli Lilly Company go about preparing the human insulin? How is the insulin thus produced different from that produced by the functional human insulin gene?

How did Eli Lilly synthesise the human insulin? Mention one difference

1.	How did Eli Lilly synthesise the human insulin? Mention one difference between this insulin and the one produced by the human pancreas.ORHow did Eli Lilly Company go about preparing the human insulin? How is the insulin thus produced different from that produced by the functional human insulin gene?
Answer» Eli Lilly prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains. Chains A and B were produced separately, extracted and combined by creating disulfide bonds to form human insulin. Insulin in human pancreas is synthesised as a pro-hormone containing the C peptide, which is removed to form mature hormone. The synthesised insulin did not contain C peptide and was directly prepared in mature form.