Step 1. Assemble your sequences. 

Put all your sequences in Fasta format in a single file. 

This file should be located in a suitably named subdirectory of your home directory on the UBiC Blast server. The DEFINITION line for each sequence should start with a unique IDENTIFIER for that sequence. 

Step 2. Convert this sequence file to a Blastable database.

The command formatdb converts your Fasta file of sequences to a set of files that can be queried with command-line BLAST. 

Step 3. Test Blast on your database.

SEE the UBiC tutorial, USING Command-line BLAST. In the blastall command-line

you will NEED to specify the location of your database by typing: -

d /disk2/home/myhome/blastdbs/custom.aa.

Step 1. Assemble your sequences. 

Put all your sequences in Fasta format in a single file. 

This file should be located in a suitably named subdirectory of your home directory on the UBiC Blast server. The definition line for each sequence should start with a unique identifier for that sequence. 

Step 2. Convert this sequence file to a Blastable database.

The command formatdb converts your Fasta file of sequences to a set of files that can be queried with command-line BLAST. 

Step 3. Test Blast on your database.

See the UBiC tutorial, Using Command-line BLAST. In the blastall command-line

you will need to specify the location of your database by typing: -

d /disk2/home/myhome/blastdbs/custom.aa.