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How many statements are correct? (a) Hind II always cut DNA molecules at particular point by recognizing a specific sequence of 6 bases. (b)After the restriction endonuclease action, single stranded portions which are overhanging stretches called sticky ends because they form nucleotide bonds with their complementary cut counter part. (c)The separated bands of DNA are cut out from the agarosegel and extracted from gel piece. This step is known as spooling. (d)Taq polymerase is used between annealing and extension of r-DNA technology.A. 1B. 2C. 0D. 3 |
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Answer» Correct Answer - C (C) (a) Hind-II always cut DNA molecules at particular point by recognising a specific sequence of 6 base pairs. (b) After the restriction endonuclease action, single stranded portions which are overhanging stretches called sticky ends because they form hydrogen bonds with their complementary cut counter part. (c) The separated bands of DNA are cut out from the agarose gel and extracted from gel piece. This step is known as elution. (d) Taq polymerase is used between annealing and extension of PCR. |
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