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How Much Equilibration Time Is Required In Between Gradient Runs On A Raptor Biphenyl Column? |
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Answer» Whether you are using FULLY porous silica or SPP silica, some EQUILIBRATION time is needed between runs if you are using a GRADIENT and the amount of time is SIMILAR for both types of columns. Usually, the EQUIVALENT of 7 column (void) volumes is sufficient unless you are using an ion-pairing technique. Whether you are using fully porous silica or SPP silica, some equilibration time is needed between runs if you are using a gradient and the amount of time is similar for both types of columns. Usually, the equivalent of 7 column (void) volumes is sufficient unless you are using an ion-pairing technique. |
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