How will you avoid the growing of microbes in nutrient medium during c

1.	How will you avoid the growing of microbes in nutrient medium during culture process? What are the techniques used to remove the microbes?
Answer» The microbial growth in culture medium can be overcome by autoclaving the medium at Plant Tissue Culture II 121°C (15 psi) for 15 to 30 minutes. Chemical sterilization using chemicals, sterilizing using UV radiation. Alcoholic sterilization using ethanol, autoclaving and filtration etc., are the various techniques used to remove microbes.

How will you avoid the growing of microbes in nutrient medium during culture process? What are the techniques used to remove the microbes?

Answer»

The microbial growth in culture medium can be overcome by autoclaving the medium at Plant Tissue Culture II 121°C (15 psi) for 15 to 30 minutes.

Chemical sterilization using chemicals, sterilizing using UV radiation. Alcoholic sterilization using ethanol, autoclaving and filtration etc., are the various techniques used to remove microbes.

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How will you avoid the growing of microbes in nutrient medium during culture process? What are the techniques used to remove the microbes?

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