i. Name the organism in which the vector shown is inserted to get the

1.	i. Name the organism in which the vector shown is inserted to get the copies of the desired gene.ii. Mention the area labelled in the vector responsible for controlling the copy number of the inserted gene.iii. Name and explain the role of a selectable marker in the vector shown.
Answer» i. Escherichia coli. ii. Origin of replication or ‘ori’ controls copy number of inserted gene. iii. The selectable markers are amp^R and tet^R (resistance to ampicillin, tetracycline). Selectable markers help to select the host cells which contain the vector (transformants) and eliminate non-transformants. If a foreign DNA ligates at the BamHI site of tetracycline resistance gene in the vector pBR322, the recombinant plasmid loses the tetracycline resistance due to insertion of DNA. It can still be selected out from non-recombinant.

i. Name the organism in which the vector shown is inserted to get the copies of the desired gene.ii. Mention the area labelled in the vector responsible for controlling the copy number of the inserted gene.iii. Name and explain the role of a selectable marker in the vector shown.

Answer»

i. Escherichia coli.

ii. Origin of replication or ‘ori’ controls copy number of inserted gene.

iii. The selectable markers are amp^R and tet^R (resistance to ampicillin, tetracycline). Selectable markers help to select the host cells which contain the vector (transformants) and eliminate non-transformants. If a foreign DNA ligates at the BamHI site of tetracycline resistance gene in the vector pBR322, the recombinant plasmid loses the tetracycline resistance due to insertion of DNA. It can still be selected out from non-recombinant.

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