Identify and illustration in the following

1.	Identify and illustration in the following
Answer» (i) A-AATTC (Recognition Site of the restriction endonuclease) (ii) B-Pru II (Origin of replication) (b) Palindromic nucleotide sequence is depicted from A & C reads same on the strands when orientations of reading is kept the same. A - Coding strand B - Template strand C - PCR strand for polymerase chain reaction. PCR is used to amplify DNA segments to a large number with in a short span of time.

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(i) Arrange the following compounds in an increasing order of basic strength : `C_(6)H_(5)NH_(2), C_(6)H_(5)N(CN_(3))_(2), (C_(2)H_(5))_(2)NH and CH_(3)NH_(2)` (ii) Arrange the following compounds in a decreasing order of `pK_(b)` values: `C_(2)H_(5)NH_(2), C_(6)H_(5)NHCH_(3), (C_(2)H_(5))_(2) NH and C_(6)H_(5)NH_(2)`
Rerrange the following in an increasing order of their basic strengths: `C_(6)H_(5)NH_(2),C_(6)H_(5)N(CH_(3))_(2),(C_(6)H_(5))_(2)` NH and `CH_(3)NH_(2)`.
(b) Estimate the minimum potential difference needed to reduce `AI_(2)O_(3)` at `500^(@)C` The gibbs energy change for the decomposition reaction `2/3AI_(2)O_(3)rarr4/3AI+O_(2)`is 960 kJ (F=96500 C `mol^(-1))`

Identify and illustration in the following

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