In a class room seminar on cloning vectors, your friend asked to expla

1.	In a class room seminar on cloning vectors, your friend asked to explain the steps to introduce the plasmid DNA to a bacteria cell, Microinjection and bilestics. Answer his questions.
Answer» For this, bacterial cells must first be made ‘competent’ to take up DNA. This is done by treating them with a specific concentration of a divalent cation, such as calcium, Recombinant DNA can then be forced into such cells by incubating the cells with recombinant DNA on ice, followed by placing them briefly at 42°C (heat shock), and then putting them back on ice. This helps to introduce the plasmid DNA into bacterial cell. Microinjection – In this recombinant DNA is directly injected into the nucleus of an animal cell. Biolistics – In this cells are bombarded with high velocity microparticles of gold or tungsten coated with DNA

In a class room seminar on cloning vectors, your friend asked to explain the steps to introduce the plasmid DNA to a bacteria cell, Microinjection and bilestics. Answer his questions.

Answer»

For this, bacterial cells must first be made ‘competent’ to take up DNA. This is done by treating them with a specific concentration of a divalent cation, such as calcium, Recombinant DNA can then be forced into such cells by incubating the cells with recombinant DNA on ice, followed by placing them briefly at 42°C (heat shock), and then putting them back on ice. This helps to introduce the plasmid DNA into bacterial cell.

Microinjection – In this recombinant DNA is directly injected into the nucleus of an animal cell. Biolistics – In this cells are bombarded with high velocity microparticles of gold or tungsten coated with DNA

Discussion

No Comment Found

Related InterviewSolutions

What were the two main occupations of the Rig Vedic Aryans?
Name the two political institutions which exercised check on the powers of the King in the Rig Vedic period.
What w’as the position of women in the Rig Vedic age ?
Describe the social life of the Rig Vedic Aryans with reference to the following: (a) The Family Structure (b) Position of Women (c) Varna System
With reference to the following explain the main features of the Rig Vedic Economy: (a) Agriculture (b) Domestication of Animals (c) Trade Transactions, Crafts and Transportation
Mention any two archaeological sources to reconstruct the later Vedic Age.
Mention any two of the Ashrams in the life of a man in Aryan society.
What information do we gather from the later Vedic literature in respect of: (a) Spread of Vedic Civilization to many New Regions. (b) Role of Iron in later Vedic Economy.
Mention briefly the rituals and sacrifices of the later Vedic Aryans?
What is the message of the Bhagavad Gita?