In a variant of chymotrypsin, Asp 102 is replaced by Glu 102. Do you e

1.	In a variant of chymotrypsin, Asp 102 is replaced by Glu 102. Do you expect the enzyme to retain activity? Schematically indicate the role of amino acid residues participating in catalysis.
Answer» Negatively charged Asp 102 partially borrow hydrogen ion from His-57. His-57 attracts hydrogen ion from adjacent Ser 195 Serine 195 gets negative charge. Serine 195 makes a nucleophilic attack on the protein substrate.

In a variant of chymotrypsin, Asp 102 is replaced by Glu 102. Do you expect the enzyme to retain activity? Schematically indicate the role of amino acid residues participating in catalysis.

Answer»

Negatively charged Asp 102 partially borrow hydrogen ion from His-57. His-57 attracts hydrogen ion from adjacent Ser 195

Serine 195 gets negative charge.

Serine 195 makes a nucleophilic attack on the protein substrate.

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In a variant of chymotrypsin, Asp 102 is replaced by Glu 102. Do you expect the enzyme to retain activity? Schematically indicate the role of amino acid residues participating in catalysis.

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