In order to study the properties of membrane proteins, they are requir

1.	In order to study the properties of membrane proteins, they are required to be isolated and purified. In one such experiment, two methods were followed to isolate membrane proteins from erythrocytes. I. Varying the pH of the buffer in which the membrane fractions were isolated. The supernatant collected and analysed. Protein 'X' was found. II. Addition of detergents to the membrane preparation. Soluble fractions analysed. Protein 'Y' was found. When proteins X and Y were studied for their structural properties, it was found that polar amino acids predominated (property 1) on the surface of one of them while the non-polar on the other (property 2). Which of the following can be deduced from this? Put tick marks in the appropriate boxes.a. X is likely to be bound to membrane by electrostatic attraction. b. Y is likely to show property. 2. c. X is likely to be the integral membrane protein. d. Y is most likely to be a cytoskeletal protein. e. Protein X is likely to be present in cell exterior (exoplasmic face) and Y in the cyotsolic face.
Answer» a. X is likely to be bound to membrane by electrostatic attraction.True b. Y is likely to show property. 2. True c. X is likely to be the integral membrane protein. False d. Y is most likely to be a cytoskeletal protein. True e. Protein X is likely to be present in cell exterior (exoplasmic face) and Y in the cyotsolic face.False

In order to study the properties of membrane proteins, they are required to be isolated and purified. In one such experiment, two methods were followed to isolate membrane proteins from erythrocytes. I. Varying the pH of the buffer in which the membrane fractions were isolated. The supernatant collected and analysed. Protein 'X' was found. II. Addition of detergents to the membrane preparation. Soluble fractions analysed. Protein 'Y' was found. When proteins X and Y were studied for their structural properties, it was found that polar amino acids predominated (property 1) on the surface of one of them while the non-polar on the other (property 2). Which of the following can be deduced from this? Put tick marks in the appropriate boxes.a. X is likely to be bound to membrane by electrostatic attraction. b. Y is likely to show property. 2. c. X is likely to be the integral membrane protein. d. Y is most likely to be a cytoskeletal protein. e. Protein X is likely to be present in cell exterior (exoplasmic face) and Y in the cyotsolic face.

Answer»

a. X is likely to be bound to membrane by electrostatic attraction.True

b. Y is likely to show property. 2. True

c. X is likely to be the integral membrane protein. False

d. Y is most likely to be a cytoskeletal protein. True

e. Protein X is likely to be present in cell exterior (exoplasmic face) and Y in the cyotsolic face.False