List the steps of preparation of temporary mount of a leaf peel to obs

1.	List the steps of preparation of temporary mount of a leaf peel to observe stomata.
Answer» (i) The leaf peel of lower epidermis is taken and cut with a blade. (ii) The cut pieces are transferred into a petridish containing water. (iii) Leaf peel is stained with safranine. (iv) Leaf peel after staining (2-3 minutes) is taken with a brush and kept on a clean glass slide. (v) Mount the peel with glycerine and put coverslip on it. (vi) Remove the excess stain and glycerin with the help of blotting paper. (vii) Observe the slide under the microscope.

List the steps of preparation of temporary mount of a leaf peel to observe stomata.

Answer»

(i) The leaf peel of lower epidermis is taken and cut with a blade.

(ii) The cut pieces are transferred into a petridish containing water.

(iii) Leaf peel is stained with safranine.

(iv) Leaf peel after staining (2-3 minutes) is taken with a brush and kept on a clean glass slide.

(v) Mount the peel with glycerine and put coverslip on it.

(vi) Remove the excess stain and glycerin with the help of blotting paper.

(vii) Observe the slide under the microscope.

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List the steps of preparation of temporary mount of a leaf peel to observe stomata.

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