1.

Mention any one significant difference between each of the following :(i) Anti codon and codon (ii) Intrinsic fluorescence and extrinsic fluorescence (iii) Introns and Exons (iv) Genomic DNA library and cDNA library (v) RAM and ROM

Answer»

(i) Anti codon is the corresponding triplet sequence on the transfer RNA (tRNA) which brings in the specific amino acid to the ribosome during translation. The anti codon is complementary to the codon, that is, if the codon is AUU, then the anti codon is UAA. 

Codon is the triplet sequence in the messenger RNA (mRNA) transcript which specifies a corresponding amino acid (or a start or stop command).

(ii)

Intrinsic fluorescenceExtrinsic fluorescence
The fluorescence shown by natural compounds is known as intrinsic fluorescence.                                                                                                                                                                         
     
There are certain chemical compounds which do not fluorescence. It can be detected after coupling them with a fluorescence probe or flour. This phenomenon is called extrinsic fluorescence.

(iii)

IntronsExons
Introns are non-coding DNA base sequences, which are found between exons, but are not transcribed part of mature mRNA.Exons are coding DNA base sequences that transcribed into w/RNA and finally code for acids in the proteins.
        

(iv)

Genomic DNA Library cDNAcDNA Library
(1) It is a collection of clones that represent the complete genome of an organism.
(2) It is the mixture of genomic DNA.


 
(1) The library made from complementary or DNA (cDNA) is called cDNA library.
(2) cDNA library consists of cDNA clones prepared by using mRNA.

 

(v)

RAMROM
(1) It stores data and programme temporarily.
(2) It contains the data of operating system, software’s and other application programmes.   

 
(1)It provides a non-volatile storage of data.
(2) It loads operating system into the memory starts the computer when switched on.



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