Name and explain the role of a selectable marker in the vector shown.

1.	Name and explain the role of a selectable marker in the vector shown.
Answer» The selectable markers are amp^R (resistance to ampicillin) and tet^R (resistance to tetracycline). Selectable markers help to select the host cells which contain the vector (transformants) and eliminating non-transformants. If a foreign DNA ligates at the BamHI site of tetracycline resistance gene in the vector pBR322, the recombinant plasmid loses the tetracycline resistance due to insertion of DNA. It can still be selected out from non-recombinant.

Answer»

The selectable markers are amp^R (resistance to ampicillin) and tet^R (resistance to tetracycline). Selectable markers help to select the host cells which contain the vector (transformants) and eliminating non-transformants.

If a foreign DNA ligates at the BamHI site of tetracycline resistance gene in the vector pBR322, the recombinant plasmid loses the tetracycline resistance due to insertion of DNA. It can still be selected out from non-recombinant.

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Name and explain the role of a selectable marker in the vector shown.

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