PCR is meant for making the multiples copies of the gene of interest.1

1.	PCR is meant for making the multiples copies of the gene of interest.1. Give the major step involved in PCR.2. Name the thermo stable DNA polymerase enzyme is used in this technique. Why?
Answer» 1. The major step involved in PCR: Denaturation – It involves the separation of DNA strands Annealing – The double strands are synthesised from free nucleotides by the action of DNA polymerase. Extension – The length of the strands are increased as a result of addition of more and more nucleotides. Thus DNA amplied up to 1 billion copies. 2. Taq polymarase PCR cylinder works at high temperature. Hence thermo stable enzyme is used.

PCR is meant for making the multiples copies of the gene of interest.1. Give the major step involved in PCR.2. Name the thermo stable DNA polymerase enzyme is used in this technique. Why?

Answer»

1. The major step involved in PCR:

Denaturation – It involves the separation of DNA strands

Annealing – The double strands are synthesised from free nucleotides by the action of DNA polymerase.

Extension – The length of the strands are increased as a result of addition of more and more nucleotides. Thus DNA amplied up to 1 billion copies.

2. Taq polymarase

PCR cylinder works at high temperature. Hence thermo stable enzyme is used.

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PCR is meant for making the multiples copies of the gene of interest.1. Give the major step involved in PCR.2. Name the thermo stable DNA polymerase enzyme is used in this technique. Why?

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