1.

Plasmid is a boon to biotechnology. Justify this statement quoting the production of human insulin as an example.

Answer»

Plasmids are extra-chromosomal, self-replicating, usually circular, double-stranded DNA molecules found naturally in many bacteria. 

In 1983, Eli Lilly an American company, first prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains. These chains A and B were produced separately, extracted and combined by creating disulfide bonds to form human insulin (humulin).



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