Question : A Danish Physician, Christian Gram developed a staining procedure to differentiate bacteria. List the various steps involved in that procedure.

Question : A Danish Physician, Christian Gram developed a staining pro

1.	Question : A Danish Physician, Christian Gram developed a staining procedure to differentiate bacteria. List the various steps involved in that procedure.
Answer» SOLUTION :(a) (i) Gram staining Techniques : 1. Prepare a smear of bacterial culture 2. Stain with crystal violet for 30 seconds 3. RINSE in distilled water for 2 seconds 4. Grams lodine for 1 minute 5. Rinse in distilled water 6. Wash in `95%` ethanol or acetone for 10 to 30 seconds 7. Rinse in distilled water 8. SAFRANIN for 30-60seconds 9. Rinse in distilled water and blot 10. Observe under MICROSCOPE (ii)