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Question : A DNA template plus primer with the structure (where =a phosphate group ) is placed in an in vitro DNA synthesis systemcontaining Mg^(2+) , an excess of the four deoxyribonucleoside triphosphates, etc. and a mutant formof E. coli DNApolymerase I that lacks 5'rarr 3' exonuclease activity . The 5' rarr 3'polymerase and 3' rarr 5'exonuclease activities of this aberrant enzyme are identical to thisaberrant enzyme are identicalto thoseof normal E. coli DNA polymerase I. It simply has no 5'rarr 3' exonuclease activity . What will be the structure of the final product ? |
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