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Question : (a) Identify the selectable markersin the diagram of E. Coli vectior shown above . ( b) How is the coding squence of alpha- galactosidase consider a better markers than the ones identified by you in the diagram ? Explain. |
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Answer» Solution :Selectable markers are TWO antibiotic- resistant genes. `"TET^(R)` = Tetracycline - resisttant GENE and `AMP^(R)` = Ampicllin - resistant gene. `beta` - galactosidas marker provides of `beta`- galactosiidase when a chromogenic substrate is added in the PLATING medium. |
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