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Question : A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reasons?

Answer»

SOLUTION :The reasons may be:
(a) DNA fragments have got completely degraded by enzymes (EXO or endo nucleases or both) which may be present in the solution.
(B) UV radiations were not used.
(c) Electrodes are not fitted properly


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