Question : A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain

Question : A plasmid DNA and a linear DNA (both are of the same size)

1.	Question : A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain
Answer» Solution :PLASMID DNA is circular, when CUT by endotlllclease it BECOMES linear but is not divided. into segments. Therefore, it forms a single band on agarose gel. A linear DNA will get cut into TWO segments by endonuclease and will form two bands on agarose gel