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Question : A plasmid has two antibiotic-resistance genes, one for tetracycline. It is treated with a restriction enzyme that cuts in the middle of the ampicillin gene. DNA fragments containing a haemoglobin gene were cut with the same enzyme. The plasmids and fragments are mixed, treated with ligase and used to transform bacterial cells. Clones that have taken up the recombinant DNA are the ones that

Answer»

can GROW on plates with both ANTIBIOTICS
can grow on plates with ampicillin but not with tetracycline
can grow on plates with tetracycline but not with ampicillin
cannot grow with any ANTIBIOTIC

Answer :C


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