Question : A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment, i.e. bacterial transformation ?

Question : A recombinant DNA molecule was created by ligating a gene t

1.	Question : A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment, i.e. bacterial transformation ?
Answer» Solution :The experiment will not likely to be AFFECTED as recombinant DNA molecule is CIRCULAR closed, with no free ends. Hence, it will not be a substrate for exonuclease enzyme which removes NUCLEOTIDES from the free ends of DNA.