Question : A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment, i.e., bacterial transformation?

Question : A recombinant DNA molecule was created by ligating a gene t

1.	Question : A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment, i.e., bacterial transformation?
Answer» Solution :The experiment will not be affected because the DNA of RECOMBINANT plasmid is circular, Le., without FREE ends, and EXONUCLEASE removes NUCLEOTIDES from the free ends of DNA.