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Question : A segment of DNA has a total of 1500 nucleotides, out of which 280 are adenine containing nucleotides. With suitable reasons and step by step calculation, determine the number of pyrirnidines bases possessed by this segment of DNA.

Answer»

SOLUTION :Ratio of PURINES to pyrimidines is equal according to Chargaff RULE, number of adenine (A) will be equal to the number of thymine (T)/A=T=280, G + C = Total number of nucleotides - Nucleotides CONTAINING A and T nitrogenous bases = 1500 - 560 = 940, number of cytosine (C) will be equal to the number of guanine (G), G = C = 470, the number of pyrimidines that the segment possess is C + T = 280 + 470 = 750


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