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InterviewSolution
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Question : Any recombinant DNA with desired gene is required in billon copies for commercial uses. How is this amplification done? Mention its steps. |
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Answer» Solution :AMPLIFICATION is the SYNTHESIS of MULTIPLE copies of gene of interest in vitro using two sets of primers and DNA polymerase enzyme. It employs PCR (Polymerase Chain Reaction). PCR is the repeated cycling of FOLLOWING three steps: 1. Denaturation 2. Annealing 3. Extension |
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