Question : Briefly explaing the 'cutting' and 'ligation' of DNA during recombinant DNA technology or how is recombinant DNA produced ?

Question : Briefly explaing the 'cutting' and 'ligation' of DNA during

1.	Question : Briefly explaing the 'cutting' and 'ligation' of DNA during recombinant DNA technology or how is recombinant DNA produced ?
Answer» Solution :.Cutting. of DNA is by RESTRICTION ENZYMES. Purified DNA molecules are incubated with restriction ENDONUCLEASES to make the .cut. . This pro-cess is repeated in vector also . The .ligation. of DNA involves several processes. The cut out .GENE of inter-est. fromthe SOURCE DNA and the cut vector with space are mixed and DNAligase is added . This result in the preparation of recombinant DNA .