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Question : Can we use recent molecular tools to identify and classify organisms? |
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Answer» Solution :The RECENT molecular taxonomical tools can be used to identify and classify the organism. The following molecular techniques and approaches are used in molecular tools. (a) DNA bar coding- Short genetic marker in an organism's DNA to identify whether it belongs to a particular species. (b) DNA hybridization - Measures the degree of genetic similarity between pools of DNA sequences. (c ) DNA finger printing- to identify an individual from a sample of DNA by looking at unique patterns in their DNA. (d) Restriction FRAGMENT Length Polymorphism (RFLP) Analysis- difference in homologous DNA sequences can be detected by the presence of fragments of different LENGTHS after DIGESTION of DNA samples. (e ) Polymerase chain reaction (PCR) sequencing- to amplify a specific GENE, or portion of gene. |
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