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Question : Classify restriction enzyme based on their mode of action.

Answer»

Solution :BASED on their mode of action restriction ENZYME are classified into Exonucleases and Endonuclearses.
a. Exonucleases are enzymes which remove nucleotides one at atime from the end of a DNA molecule .e.g Bal 31 and Exnuclease III.
b. Endonucleases are enzyme which break the internal phosphodiester BONDS within a DNA molecule e.g. Hind II , EcoRI , Pvul , BAMHI amd TaqI.


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