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Question : Derive the protocol for micropropagation of bananas.

Answer»

Solution :MICROPROPAGATION protocol for banana
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In vitro micropropagation of banana (Musa sp).
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Suckers SURFACE sterilized with 1%
NaOCl for 30 minutes
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APICAL meristems were isolated - CULTURED on
MS basal medium supplemented with
Benzylaminopurine (BAP) 10.0 mg/1 and
Indole Acetic Acid (IA A) 1.0 mg/1
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Induction of shoots within 168 days
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Kinetin 2.0 mg/1 and Naphthalene Acetic Acid
(NA A) 0.5 mg/1 for root induction
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Acclimatization in polyhouse
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Followed by shade-house under
50% light for hardening process
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Tested for genetic uniformity
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Transferred to field


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