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Question : Describe the steps of PCR technique. |
Answer» Solution :There are THREE steps of PCR technique, viz. HEAT denatura- tion, annealing and polymerization. These steps are described in the following lines :  (1) Heat DENATURATION : In heat denaturation, the DNA is heated at `91^(@)C`. Owing to heating, the hydrogen bonds in the DNA molecule are broken. This results in the formation of ss DNA. (2) Annealing: During annealing or DNA melting ss DNA is paired with primers. The primers are designed as PER the requirements. This step is carried out at `55^(@)C`. (3) Polymerization: In this step, the temperature is RAISED to `72^(@)C`. The taq polymerase is added. This helps in adding dNTPs behind the primer on the ss DNA. |
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