Question : Describe the technique which is used in the separation and isolation of DNA fragments to be used in recombinant DNA technology.

Question : Describe the technique which is used in the separation and

1.	Question : Describe the technique which is used in the separation and isolation of DNA fragments to be used in recombinant DNA technology.
Answer» Solution :DNA FRAGMENTS are negatively changed, they move towards the anode under the electric field through the medium and separate according to their size due to the SIEVING effect of AGAROSE gel. The separated fragments can be visualised by staining the DNA with ethidium bromide followed by exposure to UV radiation. Elution is the PROCESS in which the separated bands of DNA are cut out from the gel and extracted.