InterviewSolution
Saved Bookmarks
| 1. |
Question : Diagram shows a typical agarose gel showing migration of DNA fragments . a. Which of the bands has the largest and smallest DNA fragments ?b. Howcan you make fragments of DNA for electrophoresis?c.Explain separation of DNA fragments using electrophoresis.d.Point out a methodto visualize the separatedDNA fragments after electrophoresis . |
|
Answer» Solution :a. .a. is largest and .d. is smallest . b. USING restriction endonucleases c. DNA fragments are NEGATIVELY charged molecules, They are forced to MOVE towards anode under an electric field . DNA fragments SEPARATE according to their size . d. They are VISUALISED only after staining the DNA with a compound known as ethidium bromide followed by exposure UV light |
|