1.

Question : Diagram shows a typical agarose gel showing migration of DNA fragments . a. Which of the bands has the largest and smallest DNA fragments ?b. Howcan you make fragments of DNA for electrophoresis?c.Explain separation of DNA fragments using electrophoresis.d.Point out a methodto visualize the separatedDNA fragments after electrophoresis .

Answer»

Solution :a. .a. is largest and .d. is smallest .
b. USING restriction endonucleases
c. DNA fragments are NEGATIVELY charged molecules, They are forced to MOVE towards anode under an electric field . DNA fragments SEPARATE according to their size .
d. They are VISUALISED only after staining the DNA with a compound known as ethidium bromide followed by exposure UV light


Discussion

No Comment Found