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Question : DNA caused the Discuss the significance of heavy isotope of nitrogen in the Meselson and Stahl's experiment. |
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Answer» Solution :Matthew Meselson and Franklin Stahl performed the following experiment in 1958. They grew E. coli in a medium CONTAINING `""^(15)NH4Cl (""^(15)N)` is the heavy isotope of nitrogen) as the only nitrogen source for many generations. The result was that `""^(15)N` was incorporated into NEWLY synthesised DNA (as WELL as other nitrogen containing compounds). This heavy DNA molecule could be distinguished from the normal DNA by CENTRIFUGATION in a cesium chloride (CSCI) density gradient `""^(15)N` is not a radioactive isotope, and it can be separated from `""^(14)N` only based on densities). |
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