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Question : DNA library prepared by"Teminism" process or cDNA library. |
| Answer» SOLUTION :(1)cDNA is a complementary DNA which is PRODUCED USING m-ENA in the process of reserve transcription.This process is called terminism.cDNA library is constructed for eukaryotic organisms.(2)Some of the eukaryotic sequences are used for coding while some are not used for coding(non-coding).(3)In eukaryotic cells.Only those MRNA which have transcribed coding sequences are translated into protents.mRNA of different cells,tissue and organs at diffrent times and PHASES in the life cycle of an organism are isolated and converted into cDNA by the process of reverse transcription.For this process,the enzyme reverse transcriptase is used.(4)It also gives the bandings for restriction enzymes which are used.(5)This helps in identifying the exact DNA structure of viruses or plasmids. | |