Question : Explain Kuhne's experiment and the action of enzyme zymase.

1.	Question : Explain Kuhne's experiment and the action of enzyme zymase.
Answer» Solution :DEMONSTRATION of alcoholic fermentation : Take a Kuhne'sfermentation tubewhichconsistsof anuprightglass tubewith sidebulb .Pour10 % sugarsolutionmixedwithbaker'syeastinto thefermentationtubethe sidetube is filledplugthe mouthwithlid . After some time ,theglucose colution will befermented. The solution willgiveout an alcoholic smell and levelof solution in glass column will FALL due to the accumulationof `CO_(2)`GAS . It is dueto the presenceof zymasa enzyme in yeast whichconverts the GLUCOSE solution into ALCOHOL and `CO_(2)`. Nowintroduce a pellet of of KOHintothe tube ,the KOH will absorb`CO_(2)` and the levelof solution will risein upright tube .

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