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InterviewSolution
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Question : Give general information about origin of recombinant DNA molecule. |
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Answer» Solution :The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of Salmonella typhimurium. Stanley Cohen and Herbert Boyer accomplished this in 1972. The cutting of DNA at specific locations became POSSIBLE with the discovery of the so called molecular scissors - restriction enzymes. The piece of DNA was then linked with the plasmid DNA. These plasmid DNA ACT as vectors to transfer the piece of DNA attached to it. You probably know that mosquito acts as an INSECT vector to transfer the malarial parasite into human body. In the same way, a plasmid can be USED as vector to deliver an allen piece of DNA into the host organism. The linking of antibiotic resistance gene with the plasmid vector became possible with the enzyme DNA ligase. This makes a new combination of circular autonomously replicating DNA created in vitro and is known as recombinant DNA. When this DNA is transferred into Escherichia coli, a bacterium closely related to salmonella, it could replicate using the new host.s DNA polymerase enzyme and make MULTIPLE copies. |
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