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Question : Given below are the different steps in recombinant DNA technology. Arrange them according to the sequence of occurrence .a. Transferring the recombinant DNAinto the hostb. Extractionof the productc. Fragmentation of DNA by restriction endonucleasesd. Ligation of DNA fragment into a vectore. Isolation of DNAf. Culturing the host cells in a medium at large scale .g. Isolation of a desired DNA fragment . |
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Answer» SOLUTION :a. Isolation of DNA b. Fragmentation of DNA by restriction endonucleases c. Isolation of a desired DNA FRAGMENT. d. Ligation of DNA fragment into a vector e. Transferring the recombinant DNA into the host . f. Culturing the host cells in a MEDIUM at large scale . g. EXTRACTION of the PRODUCT . |
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